[email protected] (X.W.) College of Life Science, Shanxi University, Taiyuan 030006, Shanxi, China; [email protected] Correspondence: [email protected]; Tel.: +86-10-62731998; Fax: +86-10-Academic Editor: Ting Zhou Received: 16 August 2016; Accepted: 21 September 2016; Published: 24 SeptemberAbstract: This study was conducted to investigate the toxic effects of aflatoxin B1 (AFB1 ) and evaluate the effects of sporoderm-broken spores of Ganoderma lucidum (SSGL) in relieving aflatoxicosis in broilers. A total of 300 one-day-old male Arbor Acre broiler chickens were randomly divided into 4 dietary treatment options; the treatment diets have been: Manage (a basal eating plan containing regular peanut meal); AFB1 (the basal diet program containing AFB1 -contaminated peanut meal); SSGL (basal diet program with 200 mg/kg of SSGL); AFB1 +SSGL (supplementation of 200 mg/kg of SSGL in AFB1 diet plan). The contents of AFB1 in AFB1 and AFB1 +SSGL diets have been 25.0 /kg within the starter period and 22.five /kg in the finisher period. The outcomes showed that diet program contaminated having a low degree of AFB1 significantly decreased (p 0.05) the typical everyday feed intake and average daily get through the complete experiment and reduced (p 0.05) serum contents of total protein IgA and IgG. Moreover, a dietary low degree of AFB1 not simply enhanced (p 0.05) levels of hydrogen peroxide and lipid peroxidation, but in addition decreased (p 0.05) total antioxidant capability, catalase, glutathione peroxidase, and hydroxyl radical scavenger activity inside the liver and spleen of broilers. Furthermore, the addition of SSGL to AFB1 -contaminated diet regime counteracted these negative effects, indicating that SSGL includes a protective effect against aflatoxicosis. Key phrases: spores of Ganoderma lucidum; oxidative pressure; aflatoxins; antioxidant capability1. Introduction Aflatoxins (AFBs) would be the most common mycotoxins and are mainly created by Aspergillus flavus and Aspergillus parasiticus [1]. Among each of the AFBs, aflatoxin B1 (AFB1 ) is the most very toxic contaminant in foods and feedstuffs, and is classified by the International Agency of Investigation on Cancer (IARC) as a Group 1 carcinogen. In poultry, consumption of AFB1 can cause big financial losses by retarding animal growth, increasing feed efficiency and also the incidence of illness, and inducing damage to organs which include the liver and spleen [2,3]. Additionally, the carry-over of AFB1 via animal-derived merchandise in to the human meals chain can be a potential threat to human wellness as a result of its hepatotoxicity, carcinogenicity, mutagenicity, teratogenicity, and immunosuppression [4,5]. AFB1 could be the most widespread oxidative agent on the AFBs [6], and it was reported that the toxic effects of AFB1 were intimately linked with its pro-oxidant which could induce the generation of reactive oxygenToxins 2016, eight, 278; doi:10.56946-65-7 site 3390/toxins8100278 www.7-Amino-4-bromoisoindolin-1-one Chemscene mdpi.PMID:35901518 com/journal/toxinsToxins 2016, eight,2 ofspecies (ROS) and lead to the impairment of DNA, RNA, proteins, lipids, and other molecules [7,8]. Hence, some research recommended that the addition of antioxidants to diets could safeguard animals against AFB1 -induced toxicity by enhancing the antioxidant program and immunity [9,10]. Ganoderma lucidum (G. lucidum), a conventional folk medicinal mushroom, has been utilized as a vital longevity and health-promoting herb for much more than 2000 years in China [11]. The spores of Ganoderma lucidum (SGL), ejected from the pileus of G. lucidum within the mature phase, are tiny and mist-like particles of about 6.