1 mM N-[6-(biotinamido) hexyl]-3-(2-pyridyldithio) propionamide (biotin-HPDP) and
1 mM N--3-(2-pyridyldithio) propionamide (biotin-HPDP) and 0.1 mM ascorbate, and incubating at area temperature for 1 h. Then proteins had been precipitated with two vols of ?0 acetone. Biotin-labelled proteins…